Coding
hACE2 B.S

Part:BBa_K3653009:Design

Designed by: Shay Bamany, Shany Greenstein, Dor Josef, Amir Betker   Group: iGEM20_Technion-Israel   (2020-10-11)


Human ACE2 (codon optimized for B.Subtilis)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1024
    Illegal EcoRI site found at 2214
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1024
    Illegal EcoRI site found at 2214
    Illegal NheI site found at 2491
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1024
    Illegal EcoRI site found at 2214
    Illegal BglII site found at 2476
    Illegal XhoI site found at 27
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1024
    Illegal EcoRI site found at 2214
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1024
    Illegal EcoRI site found at 2214
    Illegal NgoMIV site found at 1685
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 101


Design Notes

Codon optimized for B.Subtilis


Source

Based on the ACE2 gene from the human genome - found in the article: https://www.ncbi.nlm.nih.gov/gene/59272


References